Peptide Purity
How to Achieve and Verify Peptide Purity
The peptides provided here at Pharma Lab Global are peptides with a purity of more than ninety-nine per cent. Pharma Lab Global manages to offer the finest quality proteins and peptides by combining solid phase peptide synthetic techniques with modern technological solutions. Their peptides are, thus, fit for use in any research study or any lab application.
Peptide purity and verification is something that can only occur via the utilization of uncompromised peptide manufacture and production process. Implementation of both mass spectrometry analysis and high-performance liquid chromatography, as well as top quality control measures, should then back the two.
Mass Spectrometry and HPLC
HPLC or high-performance liquid chromatography refers to a scientific method often used in the separation, identification, and quantification of all the components contained in a mixture. The process is naturally superior and which allows for highly accurate peptide measurement to be achieved.
MS or mass spectrometry, on the other hand, is a method utilized when measuring masses in a sample. It does so by ionizing all the chemical classes and then proceeding to sort the available ions according to their mass to charge proportion. An ion signal is used to plot the results as a function of this mass to charge percentage.
The two methods are highly accurate and reliable, and they are the most commonly used when it comes to testing peptides, scientifically proving their purity, and in peptides identification.
We at Pharma Lab Global take a lot of pride in the quality of all products manufactured by our team. To guarantee that peptide purity isn’t affected in any way, peptide testing is conducted at all production stages inside our peptide synthesis laboratory. Here, peptide sequential fingerprints also get checked for their precision accuracy.
What Is the Ideal Peptide Purity Level?
Researchers have completed x-ray diffraction studies of numerous tiny peptides to help them determine the physical attributes possessed by peptide bonds. The studies have shown that peptide bonds are planer and rigid.
The physical appearances are predominantly a consequence of the amide resonance interaction. Amide nitrogen is in a position to delocalize its singular electrons pair into the carbonyl oxygen. The resonance has a direct effect on the peptide bond structure.
Undeniably, the N-C bond of each peptide bond is, in fact, shorter compared to the N-Ca bond. It also happens that the C=0 bond is lengthier compared to the ordinary carbonyl bonds.
The amide hydrogen and the carbonyl oxygen in a peptide are in a trans configuration, as opposed to being in a cis configuration. A trans configuration is considered to be more dynamically encouraging because of the possibility of steric interactions when dealing with a cis configuration.
Peptide Bonds and Polarity
Pharma Lab Global only provides the highest purity levels possible, with all its peptides having a purity percentage that exceeds ninety-nine per cent. Peptides on sale on our platform can be used for both developmental and research purposes. But then again, many manufacturers tend to sell peptides having different purity levels, which are intended for use in research activities.
It’s not uncommon for researchers to start wondering what’s the least acceptable peptide purity level for use in different scenarios. Typically, the purer it is, the more favourable its preparation. Essentially, when it comes to applications such as clinical trials and vitro studies, only peptides having a purity level of more than ninety-eight per cent ought to be used.
Nevertheless, there are specific scenarios where it may make sense to use peptides having a lower purity level. It all comes down to the kind of application that the researcher has in mind for the peptides. Below is a look at the least acceptable purity levels.
>95% Peptide Purity (Highly Pure)
As mentioned earlier, this is the highest purity level and whose peptides are meant for use in particular applications. While they are costlier, they are ideal for different types of studies, as shown below. It’s a level meant for use by scientists and researchers conducting sensitive studies.
- Chromatography standard
- In vivo and in vitro studies
- Quantitative RIA and ELISA protocol standard
- Clinical trials
- Competitive inhibition and quantitative blocking studies
- Studies where peptides have been used as medications
- Quantitative enzyme and protease studies
- Cosmetic peptides for cosmeceuticals
- Monoclonal antibody production
- Crystallography
>85% Peptide Purity (Mid-range)
Mid-range peptides are mainly used for analytical applications. They are peptides whose purity levels range between seventy and eighty-five per cent. Generally, researchers use them to produce, purify, and test antibodies. Peptides in this class can also be used in semi-quantitative and biochemistry applications.
Typical applications are as shown below:
- Biological activity testing
- Peptide blocking studies
- Epitome mapping tests
- Phosphorylation studies
- Semi-quantitative studies touching on enzyme-substrate interactions
- NMR studies
- Cell attachment studies
>70% Peptide Purity (Lower Levels)
The lowest purity preparation for peptides is between fifty and seventy per cent, with most peptides recording an average of around sixty per cent purity level. Peptides falling in this category are referred to as “desalted” or “crude” peptides. While the name used to refer to them is not flattering, they do have a use as seen below.
- Antigens for affinity purification or polyclonal antibody production
- Conducting an ELISA standard test used in measuring antibody titers
- Peptide arrays
Peptides having a higher purity level are ideal for use in research studies and applications that may have a low acceptable peptide purity level. As mentioned earlier, Pharma Lab Global prides itself in being able to provide high peptide purity levels that not only meet but also exceed, purity requirements stated previously.
